Understanding the switchable solvent extraction of phospholipids from dairy byproducts

Dairy byproducts represent a rich source of phospholipids with the potential for isolation and further commercialization. In this study, we extracted from two dairy (buttermilk beta-serum) using tertiary amine (N,N-dimethylcyclohexylamine, CyNMe2) as switchable hydrophilicity solvent. For comparison, were via Folch (chloroform: methanol). CyNMe2 extraction resulted in recovery values 98.66 ± 0.89 7.67 0.51% buttermilk beta-serum, respectively. Insights into mechanism obtained by analysis protein profile, particle size, zeta potential, microstructure. generated microfractures hollow openings solid matrix through ion pair formation that releases matrix. comparison to Folch, higher concentration (up 9-fold increment), it provided different relative distribution, where phosphatidylcholine phosphatidylinositol predominant phospholipids. The outcomes study help gain insights which acts, develop strategies byproducts.